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Spore-Forming Probiotics and Their Mechanisms of Action: A Particular Focus on Alkalihalobacillus clausii

Abstract Gut microbiota play crucial roles in host health, including immune regulation, metabolism, and nutrient absorption. Its dysregulation has been linked to various diseases. When administered in sufficient amounts, probiotics can contribute to restoring the gut microbial balance and maintain homeostasis. Species of the genus Bacillus and related genera (Alkalihalobacillus and Heyndrickxia) have been extensively studied and commercialized as probiotics due to their ability to form endosp...

Synergy Between the Auranofin Analogue PEt3AuCl and Membrane Disruptors, Efflux-Pump Blockers, and Glutathione-Depletors Uncovers Tolerance Pathways in Pseudomonas aeruginosa

Auranofin (AF), an FDA-approved drug for rheumatoid arthritis, exhibits strong antibacterial activity against Gram-positive bacteria, while Gram-negative species remain largely tolerant. This study assessed the antimicrobial activity of AF and three analogues against clinically relevant Gram-negative pathogens and explored tolerance mechanisms in Pseudomonas aeruginosa. Broth microdilution assays were performed on reference strains and clinical isolates of Escherichia coli, Klebsiella pneumon...

In-depth Microbiological Characterization of Urine From Subjects With Type 2 Diabetes

Context Lower urinary tract symptoms (LUTS) are common in type 2 diabetes (T2D), affecting quality of life and potentially leading to medication discontinuation. Among various factors contributing to LUTS, recent observations suggest a critical role of the urinary microbiota. Research on urinary dysbiosis in T2D remains underexplored.Objective We conducted a pilot study to investigate differences in the urinary microbiota between patients with T2D and healthy individuals and its potential ind...

A Millifluidic Chamber for Controlled Shear Stress Testing: Application to Microbial Cultures

In vitro platforms such as bioreactors and microfluidic devices are commonly designed to engineer tissue models as well as to replicate the crosstalk between cells and microorganisms hosted in the human body. These systems promote nutrient supply and waste removal through culture medium recirculation; consequently, they intrinsically expose cellular structures to shear stress, be it a desired mechanical stimulus to drive the cell fate or a potential inhibitor for the model maturation. Assessi...

Study of the Adhesion of the Human Gut Microbiota on Electrospun Structures

Although the adhesion of bacteria on surfaces is a widely studied process, to date, most of the works focus on a single species of microorganisms and are aimed at evaluating the antimicrobial properties of biomaterials. Here, we describe how a complex microbial community, i.e., the human gut microbiota, adheres to a surface to form stable biofilms. Two electrospun structures made of natural, i.e., gelatin, and synthetic, i.e., polycaprolactone, polymers were used to study their ability to bot...

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Prodotti recenti

Spore-Forming Probiotics and Their Mechanisms of Action: A Particular Focus on Alkalihalobacillus clausii

Synergy Between the Auranofin Analogue PEt3AuCl and Membrane Disruptors, Efflux-Pump Blockers, and Glutathione-Depletors Uncovers Tolerance Pathways in Pseudomonas aeruginosa

In-depth Microbiological Characterization of Urine From Subjects With Type 2 Diabetes

A Millifluidic Chamber for Controlled Shear Stress Testing: Application to Microbial Cultures

Study of the Adhesion of the Human Gut Microbiota on Electrospun Structures

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