Abstract: Circular RNAs (circRNAs), a class of covalently closed RNAs formed by a back-splicingreaction, have been involved in the regulation of diverse oncogenic processes. In this article wedescribe circVAMP3, a novel circular RNA overexpressed in RH4, a representative cell line of alveolarrhabdomyosarcoma. We demonstrated that circVAMP3 has a differential m6A pattern opposed toits linear counterpart, suggesting that the two isoforms can be differently regulated by such RNAmodification. Moreover, we show how circVAMP3 depletion in alveolar rhabdomyosarcoma cellscan impair cell cycle progression, through the alteration of the AKT-related pathways, pointing tothis non-coding RNA as a novel regulator of the alveolar rhabdomyosarcoma progression and as aputative future therapeutic target.
CircVAMP3: A circRNA with a role in alveolar rhabdomyosarcoma cell cycle progression
Alessio Colantoni;
2021-01-01
Abstract
Abstract: Circular RNAs (circRNAs), a class of covalently closed RNAs formed by a back-splicingreaction, have been involved in the regulation of diverse oncogenic processes. In this article wedescribe circVAMP3, a novel circular RNA overexpressed in RH4, a representative cell line of alveolarrhabdomyosarcoma. We demonstrated that circVAMP3 has a differential m6A pattern opposed toits linear counterpart, suggesting that the two isoforms can be differently regulated by such RNAmodification. Moreover, we show how circVAMP3 depletion in alveolar rhabdomyosarcoma cellscan impair cell cycle progression, through the alteration of the AKT-related pathways, pointing tothis non-coding RNA as a novel regulator of the alveolar rhabdomyosarcoma progression and as aputative future therapeutic target.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
