Influence of abutment macro- and micro-geometry on morphologic and morphometric features of peri-implant connective tissue

AbstractObjectives: The aim of the present human observational study is to provide morphologic and morphometric analysis of peri-implant connective tissue next toabutments with divergent or convergent macro-geometry and different surfacemicro-characteristics.Materials and Methods: Thirty patients were rehabilitated with single implants in theposterior area and one out of three different healing abutments with a one-stagetechnique: machined divergent abutment (DIV-MAC), machined convergent abutment (CONV-MAC) or convergent abutment with ultrathin threaded surface (CONVUTM). At 3 months postimplant insertion, peri-implant soft tissue was harvested; thefollowing outcomes were investigated: histomorphometry (vertical width of connective and epithelial components) as detected by histology and polarized light; and connective tissue vertical width and 3D organization as detected by synchrotron-basedhigh-resolution phase-contrast-based tomography (PhC-μCT).Results: Significant differences in connective tissue vertical dimension (aJE-AM) werefound between DIV-MAC and both CONV-MAC and CONV-UTM, both by histologyand PhC-μCT, with significantly higher values for the last two groups. Moreover, 2Dhistological analysis did not find significant differences in the junctional epitheliumvertical dimension (PM-aJE). Importantly, PhC-μCT analysis revealed, at 3D level, significant greater amount and density of collagen bundles for CONV-UTM comparedwith the other two groups.Conclusions: Convergent abutment profiles, regardless of their surface microgeometry, seem to favor axial development of peri-implant connective tissue.Moreover, ultrathin threaded surfaces seem associated with denser and greater connective tissue organization, which might improve peri-implant soft tissue seal.