This paper describes the development of microchip electrophoresis (ME) devices for detection of nitric oxide (NO) from bulk cell lysates and single cells. Detection of NO and other endogenous electroactive species in Jurkat cells was achieved using ME with amperometric detection. Electrochemical (EC) results were then validated by ME coupled to laser-induced fluorescence (LIF) using 4-amino-5-methylamino-2',7'-difluorofluorescein diacetate (DAF-FMDA), a NO-specific probe. In parallel, a single cell analysis device was developed for the determination of intracellular NO using DAF-FMDA and LIF detection.
Microchip electrophoresis devices for the detection of nitric oxide: comparison of bulk cell and single cell analysis.
Giuseppe Caruso;
2011-01-01
Abstract
This paper describes the development of microchip electrophoresis (ME) devices for detection of nitric oxide (NO) from bulk cell lysates and single cells. Detection of NO and other endogenous electroactive species in Jurkat cells was achieved using ME with amperometric detection. Electrochemical (EC) results were then validated by ME coupled to laser-induced fluorescence (LIF) using 4-amino-5-methylamino-2',7'-difluorofluorescein diacetate (DAF-FMDA), a NO-specific probe. In parallel, a single cell analysis device was developed for the determination of intracellular NO using DAF-FMDA and LIF detection.File in questo prodotto:
Non ci sono file associati a questo prodotto.
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
